Stress proteins of Clostridium perfringens type A immunoreact with antiserum from rabbits infected with gas gangrene

Norma Heredia ^[1] ; Elva Aréchiga ^[1] ; Ronald Labbé Ronald Labbé ^[2] ; Santos García ^[1]
1. [1] Universidad Autónoma de Nuevo León
  
  Universidad Autónoma de Nuevo León
  
  México
2. [2] Food Science Department, University of Massachusetts, Amherst, USA
Localización: International microbiology: official journal of the Spanish Society for Microbiology, ISSN 1139-6709, Vol. 6, Nº. 4, 2003, págs. 259-261
Idioma: inglés
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Resumen
- Various stressors were used to induce stress proteins in Clostridium perfringens. Cultures of C. perfringens FD-1041 were subjected to cold shock (28°C for 1 h), acid shock (pH 4.5 for 30 min), or heat shock (50°C for 30 min). Cells were lysed and protein samples were analyzed by immunoblotting with antiserum derived from rabbits suffering from gas gangrene. Eight cold shock proteins (approximate Mr 101, 82, 70, 37, 22, 12, 10 and 6 kDa) and also eight heat shock proteins (approximate Mr 101, 82, 70, 27, 22, 16, 12 and 10 kDa) were immunoreactive with the serum. No immunoreactive proteins were detected in samples subjected to acid shock proteins and purified DnaKprotein was also non-immunoreactive with the serum. These immunogenic stress proteins may be important in regulating diseases caused by C. perfringens . Such proteins could be involved in cell survival mechanisms, serve as targets during infection, or play a role in recognition of the bacteria by the host.