Resistance to β-lactam antibiotics in Aeromonas hydrophila isolated from rainbow trout (Onchorhynchus mykiss)

• Maria José Saavedra ^[2] ; Sandra Guedes-Novais ^[2] ; Anabela Alves ^[2] ; Paulo Rema ^[3] ; Marta Tacão ^[1] ; António Correia ^[1] ; Antonio Martínez-Murcia; Molecular Diagnostics Center (Biomolecular Technologies, S.L.); and University Miguel Hernández, Orihuela, Alicante, Spain
  1. [1] Universidade de Aveiro

     Universidade de Aveiro

     Vera Cruz, Portugal

     
  2. [2] Department of Veterinary Sciences, CECAV-University of Trás-os- Montes e Alto Douro, Portugal
  3. [3] Department of Animal Production, University of Trás-os-Montes e Alto Douro and CIMAR, University of Porto, Portugal

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• Localización: International microbiology: official journal of the Spanish Society for Microbiology, ISSN 1139-6709, Vol. 7, Nº. 3, 2004, págs. 207-211
• Idioma: inglés
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• Resumen

  • Bacterial infections caused by members of the genus Aeromonas, with a relatively high antibiotic resistance, are among the most common and troublesome diseases of fish raised in ponds with recirculation systems. In this study, carried out at an experimental aquaculture station in northern Portugal, 51 strains identified as belonging to the genus Aeromonas were isolated from 20 rainbow trout (Oncorhynchus mykiss) skin and kidney samples, as well as from raceway water samples. Macro- and microscopic examination of the fish tissues revealed lesions or cellular alterations in skin and kidney that seemed to correlate with the presence of those isolates. The sensitivity of all isolated strains to different groups of β-lactam antibiotics (penicillins, cephalosporins, monobactams and carbapenems) was evaluated using the disc diffusion method. The highest rates of resistance were to amoxicillin, carbenicillin and ticarcillin. Unexpected resistance to imipenem, an antibiotic of clinical usage, was also detected, which suggests that resistance may have been transferred to the Aeromonas population from the environment. [Int Microbiol 2004; 7(3):207–211]