Construction of a new Streptococcus pneumoniae–Escherichia coli shuttle vector based on the replicon of an indigenous pneumococcal cryptic plasmid

• R. Muñoz ^[1] ; R. López ^[1] ; E. García ^[1]
  1. [1] Department of Molecular Microbiology, National Research Center (CSIC), Madrid, Spain
• Localización: International microbiology: official journal of the Spanish Society for Microbiology, ISSN 1139-6709, Vol. 2, Nº. 1, 1999, págs. 23-28
• Idioma: inglés
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• Resumen

  • The nucleotide sequence of a cryptic plasmid (pRMG1) isolated from a type 1 Streptococcus pneumoniae has been determined and two recombinant plasmids, pRMGE1 and pRMGE2, bearing the pRMG1 replicon have been constructed. pRMGE2 is a shuttle vector for Escherichia coli and S. pneumoniae. The important characteristics of this cloning vector are: a size of 5.5 kb including a 1.4 kb fragment of pRMG1 (containing a double-stranded replication origin and an open reading frame encoding a putative replication initiation protein), a multicloning site, two antibiotic resistance markers for selection of plasmid containing cells, and blue-white colony screening in E. coli for identification of insert-containing plasmids.