Resumen de Fluorescence Polarization as a Tool to Pinpoint Vesicle Thermal Phase Transitions
Gary A. Baker, Thomas A. Betts, Siddharth Pandey
Fluorescence polarization (FP) as a biophysical technique is simplistic in its approach and has potential for the direct determination of ligand-receptor binding and supramolecular associations. Here, students determine the gel-to-liquid transition temperature (Tm) in L-α-dimyristoylphosphatidylcholine (DMPC) model bilayer systems based on steady-state FP of a lipid-tailed fluorescent marker bound therein. First students are exposed to both organized media and emission polarization phenomena and then they apply these fundamentals to readily and accurately (±1-2 °C) determine Tm for a neat DMPC vesicular system.
