Peter Krustrup, Jens Bangsbo, Paul S. Bradley, Magni Mohr, Jesper F. Christensen, Morten B. Randers, Carlo Castagna, Luke Connolly, Sarah R. Jackman
AB Purpose: This study aimed to examine the physical capacity and physiological response to the Yo-Yo Intermittent Endurance level 2 test (IE2) for untrained individuals (UTR) and trained male soccer players (TR) and to investigate the determinants of intense intermittent exercise performance. Methods: Thirty-four healthy UTR males and 15 age-matched TR performed a maximal incremental treadmill test and a Yo-Yo IE2 test. Muscle biopsies and blood samples were obtained, and heart rate (HR) was measured before, during, and after tests. Results: UTR had a 67% lower (P < 0.01) Yo-Yo IE2 performance (665 +/- 271 vs 2027 +/- 298 m; effect size (ES), 4.8), 34% lower V[spacing dot above]O2max (P < 0.01), and 19% lower resting muscle glycogen (P < 0.05) than those of TR. Blood lactate concentration and HR during the first 560 m of the Yo-Yo IE2 test were higher (P < 0.01) in UTR than those in TR (560 m, 7.4 +/- 2.8 vs 2.4 +/- 0.8 mM; ES, 1.7-2.8; 188 +/- 11 vs 173 +/- 8 bpm; ES, 0.9-1.5), with no differences at exhaustion. Time >95% HRmax was lower (P < 0.01) in UTR than that in TR (1.0 +/- 1.1 vs 6.3 +/- 2.9 min; ES, 3.1). Mean rates of muscle creatine phosphate utilization (16.5 +/- 9.5 vs 4.3 +/- 2.7 mmol[middle dot]kg-1 d.w[middle dot]min-1), muscle lactate accumulation (16.8 +/- 9.1 vs 4.2 +/- 2.9 mmol[middle dot]kg-1 d.w.[middle dot]min-1), and glycogen breakdown (29.6 +/- 14.2 vs 7.7 +/- 5.4 mmol[middle dot]kg-1 d.w.[middle dot]min-1) were fourfold higher (P < 0.01; ES, 1.4-1.7) in UTR than those in TR. For UTR, correlations (P < 0.01) were observed between Yo-Yo IE2 performance and V[spacing dot above]O2max (r = 0.77), incremental treadmill test performance (r = 0.79), and muscle citrate synthase activity (r = 0.57) but not for TR (r = -0.12 to 0.50; P > 0.05). Conclusions: The Yo-Yo IE2 test was shown to possess high construct validity by showing large differences in performance, HR, and anaerobic metabolism between UTR and TR. In addition, V[spacing dot above]O2max seemed to be important for intermittent exercise performance in UTR but not for TR.
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