Endurance Training Attenuates Catabolic Signals Induced by TNF-[alpha] in Muscle of Mice.

• Autores: Julie Rodriguez, Rodrigo Fernandez Verdejo, Nicolas Pierre, Fabian Priem, Marc Francaux
• Localización: Medicine & Science in Sports & exercise: Official Journal of the American College of Sports Medicine, ISSN 0195-9131, Vol. 48, Nº. 2, 2016, págs. 227-234
• Idioma: inglés
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• Resumen

  • AB Purpose: To determine whether endurance training attenuates the negative effects induced by an acute injection of tumor necrosis factor-[alpha] (TNF-[alpha]) in skeletal muscle of mice. Methods: Trained (6 wk of endurance training at 60% of maximal velocity) and untrained mice were injected with TNF-[alpha] or vehicle and killed 6 h after. Tibialis anterior muscles were analyzed using Western blot and qRT-PCR for markers of inflammation and protein synthesis/degradation. Results: Independently of training, TNF-[alpha] increased the mRNA of cytokines and downregulated signals linked to protein synthesis. The phosphorylation of IKK[alpha]/[beta] and I[kappa]B[alpha] induced by TNF-[alpha] was blunted in trained mice, suggesting altered NF-[kappa]B activation. This was associated with lower induction of several markers of protein degradation (FoxO1, MURF1, MAFbx, myostatin, Gabarapl1, and LC3BII/LC3BI ratio). Conclusions: Endurance training protects skeletal muscle against the activation of protein degradation signaling pathways induced by TNF-[alpha]