Kaempferol inhibits the production of ROS to modulate OPN–αvβ3 integrin pathway in HUVECs

• Hong-Bo Xiao ^[1] ; Xiang-Yang Lu ^[1] ; Zi-Kui Liu ^[1] ; Zhi-Feng Luo ^[2]
  1. [1] Hunan Agricultural University

     Hunan Agricultural University

     China

     
  2. [2] Xiangnan University

     Xiangnan University

     China

     
• Localización: Journal of physiology and biochemistry, ISSN-e 1877-8755, ISSN 1138-7548, Vol. 72, Nº. 2 (June 2016), 2016, págs. 303-313
• Idioma: inglés
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• Resumen

  • In the present study, we tested the hypothesis that aldosterone regulates osteopontin (OPN)-related signaling pathways to promote nuclear factor κB (NF-κB) activation in primary human umbilical vein endothelial cells (HUVECs) and that kaempferol, a flavonoid compound, blocks those changes. Aldosterone induced productions of reactive oxygen species (ROS), OPN, interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) and expression of nicotinamide adenine dinucleotide phosphate-oxidase 4 (Nox4), NF-κB, OPN, alphavbeta3 (αvβ3) integrin, and inhibitor of NF-κB alpha phosphorylation (P-IκBα) in HUVEC. HUVECs were pretreated with kaempferol (0, 1, 3, or 10 μM) for 1 h and exposed to aldosterone (10−6 M) for 24 h. Kaempferol reduced ROS, OPN, NF-κB, IL-6, and TNF-α levels; Nox4, αvβ3 integrin; and P-IκBα expressions. The effect of aldosterone was also abrogated by spironolactone (10−6 M). In addition, vitamin C (20 mmol/L) reduced ROS production. Vitamin C and LM609 (10 μg/mL) treatment decreased expressions of OPN, αvβ3 integrin, and NF-κB (P < 0.05 or P < 0.01). The present results suggest that kaempferol may modulate OPN–αvβ3 integrin pathway to inhibit NF-κB activation in HUVECs.