Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis

• Yohann Boutté ^[1] ; Márcia Frescatada-Rosa ^[6] ; Shuzhen Men ^[1] ; Cheung-Ming Chow ^[2] ; Kazuo Ebine ^[3] ; Anna Gustavsson ^[6] ; Lenore Johansson ^[4] ; Takashi Ueda ^[3] ; Ian Moore ^[2] ; Gerd Jürgens ^[5] ; Markus Grebe ^[1]
  1. [1] Swedish University of Agricultural Sciences

     Swedish University of Agricultural Sciences

     Uppsala domkyrkoförs., Suecia

     
  2. [2] University of Oxford

     University of Oxford

     Oxford District, Reino Unido

     
  3. [3] University of Tokyo

     University of Tokyo

     Japón

     
  4. [4] Umeå University

     Umeå University

     Suecia

     
  5. [5] University of Tübingen

     University of Tübingen

     Landkreis Tübingen, Alemania

     
  6. [6] Department of Plant Physiology, Umeå Plant Science Centre (UPSC), Umeå University, Umeå, Sweden

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• Localización: EMBO journal: European Molecular Biology Organization, ISSN 0261-4189, Vol. 29, Nº. 3, 2009, págs. 546-558
• Idioma: inglés
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  • Cytokinesis represents the final stage of eukaryotic cell division during which the cytoplasm becomes partitioned between daughter cells. The process differs to some extent between animal and plant cells, but proteins of the syntaxin family mediate membrane fusion in the plane of cell division in diverse organisms. How syntaxin localization is kept in check remains elusive. Here, we report that localization of the Arabidopsis KNOLLE syntaxin in the plane of cell division is maintained by sterol-dependent endocytosis involving a clathrin- and DYNAMIN-RELATED PROTEIN1A-dependent mechanism. On genetic or pharmacological interference with endocytosis, KNOLLE mis-localizes to lateral plasma membranes after cell-plate fusion. Fluorescence-loss-in-photo-bleaching and fluorescence-recovery-after-photo-bleaching experiments reveal lateral diffusion of GFP-KNOLLE from the plane of division to lateral membranes. In an endocytosis-defective sterol biosynthesis mutant displaying lateral KNOLLE diffusion, KNOLLE secretory trafficking remains unaffected. Thus, restriction of lateral diffusion by endocytosis may serve to maintain specificity of syntaxin localization during late cytokinesis.