Epigenetic engineering shows H3K4me2 is required for HJURP targeting and CENP-A assembly on a synthetic human kinetochore

• Jan H Bergmann ^[1] ; Mariluz Gómez Rodríguez ^[2] ; Nuno M C Martins ^[1] ; Hiroshi Kimura ^[3] ; David A Kelly ^[1] ; Hiroshi Masumoto ^[4] ; Vladimir Larionov ^[5] ; Lars E T Jansen ^[2] ; William C Earnshaw ^[1]
  1. [1] Wellcome Trust Centre for Cell Biology

     Wellcome Trust Centre for Cell Biology

     Reino Unido

     
  2. [2] Instituto Gulbenkian de Ciência

     Instituto Gulbenkian de Ciência

     Oeiras e São Julião da Barra, Portugal

     
  3. [3] Osaka University

     Osaka University

     Kita Ku, Japón

     
  4. [4] Kazusa DNA Research Institute

     Kazusa DNA Research Institute

     Japón

     
  5. [5] National Institutes of Health

     National Institutes of Health

     Estados Unidos

     

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• Localización: EMBO journal: European Molecular Biology Organization, ISSN 0261-4189, Vol. 30, Nº. 2, 2010, págs. 328-340
• Idioma: inglés
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• Resumen

  • Here, centromeric histone marks on a human artificial chromosome are found to resemble the chromatin landscape in transcribed genes, and selective manipulation shows them to govern the incorporation of the centromere-specifying CENP-A histone variant.