Architecture of the RNA polymerase–Spt4/5 complex and basis of universal transcription processivity

• Fuensanta W Martinez-Rucobo ^[1] ; Sarah Sainsbury ^[1] ; Alan CM Cheung ^[1] ; Patrick Cramer ^[1]
  1. [1] Ludwig Maximilian University of Munich

     Ludwig Maximilian University of Munich

     Kreisfreie Stadt München, Alemania

     
• Localización: EMBO journal: European Molecular Biology Organization, ISSN 0261-4189, Vol. 30, Nº. 7, 2011, págs. 1302-1310
• Idioma: inglés
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• Resumen

  • Spt5 and NusG play a conserved role in stimulating RNA polymerase II transcription elongation and processivity. Here, the crystal structure of Spt4/5 bound to the RNA polymerase clamp domain reveals that the factor binds above DNA and RNA in the active centre cleft preventing premature dissociation of the polymerase.