Insights into Chi recognition from the structure of an AddAB-type helicase–nuclease complex

• Kayarat Saikrishnan ^[1] ; Joseph T Yeeles ^[2] ; Neville S Gilhooly ^[2] ; Wojciech W Krajewski ^[1] ; Mark S Dillingham ^[2] ; Dale B Wigley ^[1]
  1. [1] Institute of Cancer Research

     Institute of Cancer Research

     Reino Unido

     
  2. [2] University of Bristol

     University of Bristol

     Reino Unido

     
• Localización: EMBO journal: European Molecular Biology Organization, ISSN 0261-4189, Vol. 31, Nº. 6, 2012, págs. 1568-1578
• Idioma: inglés
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• Resumen

  • Homologous recombination DNA repair requires double-strand break resection by helicase–nuclease enzymes. The crystal structure of bacterial AddAB in complex with DNA substrates shows that it employs an inactive helicase site to recognize ‘Chi' recombination hotspot sequences that regulate resection.