Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase

• Shoken Lee ^[1] ; Yasunori Uchida ^[1] ; Jiao Wang ^[2] ; Tatsuyuki Matsudaira ^[1] ; Takatoshi Nakagawa ^[3] ; Takuma Kishimoto ^[4] ; Kojiro Mukai ^[1] ; Takehiko Inaba ^[4] ; Toshihide Kobayashi ^[4] ; Robert S Molday ^[2] ; Tomohiko Taguchi ^[1] ; Hiroyuki Arai ^[1]
  1. [1] University of Tokyo

     University of Tokyo

     Japón

     
  2. [2] University of British Columbia

     University of British Columbia

     Canadá

     
  3. [3] Osaka Medical College

     Osaka Medical College

     Japón

     
  4. [4] 4 Lipid Biology Laboratory, RIKEN Wako-shi, Saitama, Japan

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• Localización: EMBO journal: European Molecular Biology Organization, ISSN 0261-4189, Vol. 34, Nº. 5, 2015, págs. 669-688
• Idioma: inglés
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• Resumen

  • P4-ATPases translocate aminophospholipids, such as phosphatidylserine (PS), to the cytosolic leaflet of membranes. PS is highly enriched in recycling endosomes (REs) and is essential for endosomal membrane traffic. Here, we show that PS flipping by an RE-localized P4-ATPase is required for the recruitment of the membrane fission protein EHD1. Depletion of ATP8A1 impaired the asymmetric transbilayer distribution of PS in REs, dissociated EHD1 from REs, and generated aberrant endosomal tubules that appear resistant to fission. EHD1 did not show membrane localization in cells defective in PS synthesis. ATP8A2, a tissue-specific ATP8A1 paralogue, is associated with a neurodegenerative disease (CAMRQ). ATP8A2, but not the disease-causative ATP8A2 mutant, rescued the endosomal defects in ATP8A1-depleted cells. Primary neurons from Atp8a2−/− mice showed a reduced level of transferrin receptors at the cell surface compared to Atp8a2+/+ mice. These findings demonstrate the role of P4-ATPase in membrane fission and give insight into the molecular basis of CAMRQ.