Molecular Pathology of Pulmonary Edema in Forensic Autopsy Cases with Special Regard to Fatal Methamphetamine Intoxication
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Yu Du [1] ; Hong-Nian Jin [5] ; Rui Zhao [2] ; Dong Zhao [6] ; Ye Xue [4] ; Bao-Li Zhu [2] ; Da-Wei Guan [2] ; Xiao-Li Xie [3] ; Qi Wang [4]
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[1] National Police University of China
National Police University of China
China
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[2] University School of Forensic Medicine Shenyang Liaoning Province China
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[3] University, (Guangdong Provincial Key Laboratory of Tropical Disease Research) Guangzhou China
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[4] University Guangzhou China
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[5] Forensic Science Centre of Guangdong Provincial Public Security Department Yuexiu District Guangzhou China
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[6] University of Political Science and Law) Ministry of Education Haidian District Beijing China
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- Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 61, Nº. 6, 2016, págs. 1531-1537
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Pulmonary edema is a common finding in fatal methamphetamine intoxication. However, the underlying mechanism remains poorly understood. This study investigated the molecular pathology of alveolar damage involving pulmonary edema in forensic autopsy cases.
Seven candidate reference genes (RPL13A, YWHAZ, GUSB, SDHA, GAPDH, B2M, and ACTB) were evaluated in the lung by the geNorm module in qBaseplus software. RPL13A, YWHAZ, and GUSB were identified as the most stable reference genes. Using these validated refer- ence genes, intrapulmonary mRNA expressions of matrix metalloproteinases (MMPs), intercellular adhesion molecule-1 (ICAM-1), claudin-5 (CLDN-5), and aquaporins (AQPs) were examined. Relative mRNA quantification using TaqMan real-time PCR assay demonstrated higher expressions of all markers except for AQP-5 in fatal METH intoxication cases. These findings suggested alveolar damage and compensatory response in fatal METH intoxication cases. Systematic analysis of gene expressions using real-time qPCR is a useful tool in forensic death investigation.
