Endothelial basement membrane laminin 511 is essential for shear stress response

• Jacopo Di Russo ^[4] ; Anna‐Liisa Luik ^[4] ; Lema Yousif ^[4] ; Sigmund Budny ^[4] ; Hans Oberleithner ^[5] ; Verena Hofschröer ^[5] ; Juergen Klingauf ^[6] ; Ed van Bavel ^[1] ; Erik NTP Bakker ^[1] ; Per Hellstrand ^[2] ; Anirban Bhattachariya ^[2] ; Sebastian Albinsson ^[2] ; Frederic Pincet ^[3] ; Rupert Hallmann ^[4] ; Lydia M Sorokin ^[4]
  1. [1] University of Amsterdam

     University of Amsterdam

     Países Bajos

     
  2. [2] Lund University

     Lund University

     Suecia

     
  3. [3] 7 Laboratoire de Physique Statistique École Normale Superieure – PSL Research University Paris France; 8 CNRS UMR8550 Sorbonne Universités − UPMC Univ Paris 06 Université Paris Paris France
  4. [4] 1 Institute of Physiological Chemistry and Pathobiochemistry University of Muenster Muenster Germany; 2 Cells‐in‐Motion Cluster of Excellence University of Muenster Muenster Germany
  5. [5] 2 Cells‐in‐Motion Cluster of Excellence University of Muenster Muenster Germany; 3 Institute of Physiology II University of Muenster Muenster Germany
  6. [6] 2 Cells‐in‐Motion Cluster of Excellence University of Muenster Muenster Germany; 4 Institute of Medical Physics University of Muenster Muenster Germany

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• Localización: EMBO journal: European Molecular Biology Organization, ISSN 0261-4189, Vol. 36, Nº. 2, 2017, págs. 183-201
• Idioma: inglés
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  • Shear detection and mechanotransduction by arterial endothelium requires junctional complexes containing PECAM‐1 and VE‐cadherin, as well as firm anchorage to the underlying basement membrane. While considerable information is available for junctional complexes in these processes, gained largely from in vitro studies, little is known about the contribution of the endothelial basement membrane. Using resistance artery explants, we show that the integral endothelial basement membrane component, laminin 511 (laminin α5), is central to shear detection and mechanotransduction and its elimination at this site results in ablation of dilation in response to increased shear stress. Loss of endothelial laminin 511 correlates with reduced cortical stiffness of arterial endothelium in vivo, smaller integrin β1‐positive/vinculin‐positive focal adhesions, and reduced junctional association of actin–myosin II. In vitro assays reveal that β1 integrin‐mediated interaction with laminin 511 results in high strengths of adhesion, which promotes p120 catenin association with VE‐cadherin, stabilizing it at cell junctions and increasing cell–cell adhesion strength. This highlights the importance of endothelial laminin 511 in shear response in the physiologically relevant context of resistance arteries.