Rif1 Prevents Resection of DNA Breaks and Promotes Immunoglobulin Class Switching

• Autores: Michela Di Virgilio, Elsa Callen, Arito Yamane
• Localización: Science, ISSN 0036-8075, Vol. 339, Nº 6120, 2013, págs. 712-715
• Idioma: inglés
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• Resumen

  • DNA double-strand breaks (DSBs) represent a threat to the genome because they can lead to the loss of genetic information and chromosome rearrangements. The DNA repair protein p53 binding protein 1 (53BP1) protects the genome by limiting nucleolytic processing of DSBs by a mechanism that requires its phosphorylation, but whether 53BP1 does so directly is not known. Here, we identify Rap1-interacting factor 1 (Rif1) as an ATM (ataxia-telangiectasia mutated) phosphorylation-dependent interactor of 53BP1 and show that absence of Rif1 results in 5′-3′ DNA-end resection in mice. Consistent with enhanced DNA resection, Rif1 deficiency impairs DNA repair in the G1 and S phases of the cell cycle, interferes with class switch recombination in B lymphocytes, and leads to accumulation of chromosome DSBs.