Effects of phthalate esters on actin cytoskeleton of Py1a rat osteoblasts.

• L. Marchetti ^[1] ; M.G. Sabbieti ^[1] ; M. Menghi ^[1] ; S. Materazzi ^[3] ; M.M. Hurley ^[2] ; G. Menghi ^[1]
  1. [1] Università di Camerino

     Università di Camerino

     Camerino, Italia

     
  2. [2] University of Connecticut

     University of Connecticut

     Town of Mansfield, Estados Unidos

     
  3. [3] University "La Sapienza"

  Mostrar afiliaciones +
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 17, Nº. 4, 2002, págs. 1061-1066
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • We evaluated, by confocal laser scanning microscopy, the actin cytoskeleton of immortalized rat Py1a osteoblasts treated with phthalate esters (butyl benzyl phthalate, BBP and dibutyl phthalate, DBP), endocrine disruptors with estrogenic activity. We observed some peculiar modifications of actin cytoskeleton and cells changing from a spindle shape to a rounded form. In particular, F-actin formed thick bundles around the cell membrane but only a weak labeling was observed in rounded cells. Also influence on apoptosis and short-term effects on FGF-2 were studied. It was found that BBP and DBP exert their action in a similar way, act in a transient manner and do not induce apoptosis.