Ghrelin and adipose-derived mesenchymal stromal cells improve nerve regeneration in a rat model of epsilon-caprolactone conduit reconstruction

• Pedro Hernández-Cortés ^[1] ; Miguel Angel Toledo-Romero ^[3] ; Mario Delgado ^[2] ; Elena Gonzalez-Rey ^[2] ; Rafael Gómez Sánchez ^[1] ; Nicolás Prados-Olleta ^[4] ; José Aneiros-Fernández ^[5] ; Vicente Crespo-Lora ^[5] ; Mariano Aguilar ^[5] ; Pablo Galindo-Moreno ^[1] ; Francisco O'Valle ^[5]
  1. [1] Universidad de Granada

     Universidad de Granada

     Granada, España

     
  2. [2] Instituto de Parasitología y Biomedicina López Neyra

     Instituto de Parasitología y Biomedicina López Neyra

     Armilla, España

     
  3. [3] Virgen del Rocio University Hospital of Seville
  4. [4] Virgen de las Nieves University Hospital of Granada
  5. [5] Biopathology and Regenerative Medicine Institute (IBIMER, CIBM)

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• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 32, Nº. 6, 2017, págs. 627-637
• Idioma: inglés
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  • Objective. Attempts have been made to improve nerve conduits in peripheral nerve reconstruction. We investigated the potential therapeutic effect of adipose-derived mesenchymal cells (ASCs) and ghrelin (GHR), a neuropeptide with neuroprotective, trophic, and developmental regulatory actions, on peripheral nerve regeneration in a model of severe nerve injury repaired with nerve conduits. Material and methods. The right sciatic nerves of 24 male Wistar rats were 10-mm transected unilaterally and repaired with Dl-lactic-ε-caprolactone conduits. Rats were then treated locally with saline, ASCs, or GHR. At 12 weeks post-surgery, we assessed limb function by measuring ankle stance angle and percentage muscle mass reduction and evaluated the histopathology, immunohistochemistry, ultrastructure, and morphometry of myelinated fibers. Main Results. Rats receiving GHR or ASCs showed no significant increased functional recovery in ankle stance angle (p=0.372) but a higher nerve area (p=0.015), myelin area (p=0.046) and number of myelinated fibers (p=0.012) in the middle and distal segments of operated sciatic nerves in comparison to saline-treated control animals. Conclusion. These results suggest that utilization of ghrelin or ASCs may improve nerve regeneration using Dl-lactic-ε-caprolactone conduits