Resumen de An aflp-marker study of the vitis vinifera l. cultivar 'white riesling' comprising 86 clones to investigate the stability of clones

E.H. Rühl, U. Anhalt, Astrid Forneck

• The cultivar ?White Riesling? is like any other cultivar of Vitis vinifera L. commercially asexually propagated. Therefore, any clone is asexually derived from a single individual. Genetic changes can only occur through mutations.

  The aim of this study was to analyse the number of mutations among 86 different ?White Riesling? clones and to characterise the kinds of mutations observed. Single event mutations, which only appear once in one clone, stay for the dynamic grapevine genome. Mutations, which appear frequently in different clones, imply that the mutations could be loci-specific. To examine this subject 86 ?Riesling? clones were analysed with ten amplified fragments length polymorphism (AFLP) primer combina¬tions. Of the 305 marker bands analysed 135 bands were polymorphic. 38.5% of the polymorphic marker bands showed single event mutations and 17% were specific-loci mutations. This indicates that the grapevine genome is dynamic and not stable. Since genetic variability is important for plant breeders but clone stability necessary for grape growers and winemakers the knowledge of genome stability is essential and could be an indication of how to deal with dynamic clones during the breeding proce¬dures, like clonal selection, and maintenance breeding.