Combining polyethylene and polypropylene: Enhanced performance with PE/iPP multiblock polymers

• Autores: James Eagan, Marilyn Holt
• Localización: Science, ISSN 0036-8075, Vol. 355, Nº 6327, 2017, págs. 814-816
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • DNA charge transport chemistry offers a means of long-range, rapid redox signaling. We demonstrate that the [4Fe4S] cluster in human DNA primase can make use of this chemistry to coordinate the first steps of DNA synthesis. Using DNA electrochemistry, we found that a change in oxidation state of the [4Fe4S] cluster acts as a switch for DNA binding. Single-atom mutations that inhibit this charge transfer hinder primase initiation without affecting primase structure or polymerization. Generating a single base mismatch in the growing primer duplex, which attenuates DNA charge transport, inhibits primer truncation. Thus, redox signaling by [4Fe4S] clusters using DNA charge transport regulates primase binding to DNA and illustrates chemistry that may efficiently drive substrate handoff between polymerases during DNA replication.