Top-Down Proteomics: Turning Protein Mass Spec Upside-Down

• Autores: Jeffrey M. Perkel
• Localización: Science, ISSN 0036-8075, Vol. 349, Nº 6253, 2015, págs. 1243-1245
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Between alternative transcription start sites, alternative splicing, and posttranslational modifications, a given gene may produce dozens of protein variants, each with a different biological activity. Teasing apart those structure-function relationships requires mapping specific variants to their associated biological functions, and the tool of the trade for doing so is mass spectrometry. But not just any mass spec will do. Researchers need a holistic view of protein structure, data that is lost with the popular “bottom-up” proteomics strategy. Powered by today’s ultrahigh-resolution, high massaccuracy mass specs, protein biochemists are increasingly turning bottom-up upside-down. Their new alternative: topdown proteomics.