Growth dynamics of gut microbiota in health and disease inferred from single metagenomic samples

• Autores: Tal Korem, David Zeevi, Jotham Suez
• Localización: Science, ISSN 0036-8075, Vol. 349, Nº 6252, 2015, págs. 1101-1106
• Idioma: inglés
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• Resumen

  • Metagenomic sequencing increased our understanding of the role of the microbiome in health and disease, yet it only provides a snapshot of a highly dynamic ecosystem. Here, we show that the pattern of metagenomic sequencing read coverage for different microbial genomes contains a single trough and a single peak, the latter coinciding with the bacterial origin of replication. Furthermore, the ratio of sequencing coverage between the peak and trough provides a quantitative measure of a species’ growth rate. We demonstrate this in vitro and in vivo, under different growth conditions, and in complex bacterial communities. For several bacterial species, peak-to-trough coverage ratios, but not relative abundances, correlated with the manifestation of inflammatory bowel disease and type II diabetes.