Response to Comment on “Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling”

• Autores: Calvin H. Jan, Christopher C. Williams, Jonathan S. Weissman
• Localización: Science, ISSN 0036-8075, Vol. 348, Nº 6240, 2015, págs. 1217-1217
• Idioma: inglés
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• Resumen

  • Reid and Nicchitta propose that most cellular translation is carried out by a noncycling pool of endoplasmic reticulum (ER)–associated ribosomes. However, proximity-specific ribosome profiling data place an upper bound of about 7 to 16% on the fraction of cytosolic protein translation carried out by ribosomes accessible to ER-tethered biotin ligases. Moreover, yeast pulse-labeling experiments argue against there being a static population of ER-associated ribosomes.