Jhon Elier Arango Trillos, Cristhian Manuel Durán Acevedo, Jhon Edward Lizarazo Parada, Jorge Duarte
El congelamiento de semen bovino (criopreservanción) consiste en reducir la temperatura de las muestras seminales hasta el punto de estabilidad biológica. Este proceso de congelamiento se lleva acabo sumergiendo las muestras de semen en nitrógeno líquido (LN2). Cuando la muestra es sumergida, entra en un régimen de ebullición debido a la gran diferencia de temperatura entre la muestra y el nitrógeno líquido (LN2), causando evaporización y cristalización. Las curvas de congelamiento de muestras biológicas de semen bovino no son comunes en la literatura; sin embargo existen referencias que describen varios ritmos de congelamiento que se han realizado experimentalmente y han alcanzado resultados aceptables. El objetivo de este estudio es desarrollar un sistema automatizado de control para el congelamiento de semen bovino que pueda seguir o simular las curvas de congelamiento para una congelación optima después de la estabilización.
The freezing of bovine semen (cryopreservation) consists in reducing the temperature of the sample until the point of biological stability. This freezing process is carried out by immersing the semen samples into liquid nitrogen (LN2). When a sample is submerged, it enters into a boiling regime due to the large difference in temperatures between the sample and the liquid nitrogen (LN2), causing evaporation and crystallization. Freezing curves for biological samples of bovine semen are rarely found in scientific literature; however, there are several rhythms of freezing that have been manually carried out and have achieved acceptable results. The main objective of this study is to design and implement an automated control system of freezing bovine semen that can follow or simulate the ideal freezing curves for optimal freezing after stabilization.The freezing of bovine semen (cryopreservation) consists in reducing the temperature of the sample until the point of biological stability. This freezing process is carried out by immersing the semen samples into liquid nitrogen (LN2). When a sample is submerged, it enters into a boiling regime due to the large difference in temperatures between the sample and the liquid nitrogen (LN2), causing evaporation and crystallization. Freezing curves for biological samples of bovine semen are rarely found in scientific literature; however, there are several rhythms of freezing that have been manually carried out and have achieved acceptable results. The main objective of this study is to design and implement an automated control system of freezing bovine semen that can follow or simulate the ideal freezing curves for optimal freezing after stabilization.
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