Two basic assumptions are frequently used in the measurement of testis volume: 1) they are highly conserved and thus measures are frequently restricted to a measure of a single testis/individual, and 2) a prolate spheroid provides an adequate measure of volume. Based on the measurement of museum and road kill Pogona barbata (Eastern bearded dragon), we showed these assumptions did not hold and recommend that when considering testis volume, researchers measure both testes and the formula used should be informed by careful consideration of their shape.
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