Evaluation of serum IgE in peach-allergic patients with systemic reaction by using recombinant Pru p 7 (gibberellin-regulated protein)

• Y. Mori ^[1] ; F. Okazaki ^[2] ; C. Inuo ^[1] ; Y. Yamaguchi ^[6] ; S. Masuda ^[3] ; S. Sugiura ^[7] ; T. Fukuie ^[4] ; M. Nagao ^[8] ; I. Tsuge ^[1] ; T. Yosikawa ^[1] ; A. Yagami ^[1] ; K. Matsunaga ^[1] ; T. Fujisawa ^[8] ; K. Ito ^[9] ; H. Narita ^[5] ; Y. Kondo ^[1]
  1. [1] Fujita Health University

     Fujita Health University

     Japón

     
  2. [2] Ryukoku University

     Ryukoku University

     Kamigyō-ku, Japón

     
  3. [3] Kyoto University

     Kyoto University

     Kamigyō-ku, Japón

     
  4. [4] Hamamatsu University

     Hamamatsu University

     Naka-ku, Japón

     
  5. [5] Kyoto Women's University

     Kyoto Women's University

     Kamigyō-ku, Japón

     
  6. [6] Kyoto College of Nutritional and Medical Science, Kyoto, Japan
  7. [7] Aichi Children's Health and Medical Center, Japan
  8. [8] Mie National Hospital, Japan
  9. [9] Aichi Children's Health and Medical Center, Aichi, Japan

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• Localización: Allergologia et immunopathologia: International journal for clinical and investigate allergology and clinical immunology, ISSN-e 1578-1267, ISSN 0301-0546, Vol. 46, Nº. 5, 2018, págs. 482-490
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Background Lipid transfer protein (LTP) is a major fruit allergen. It has, however, recently been revealed that the systemic reaction in peach-allergic patients is related not only to LTP (Pru p 3) but also to gibberellin-regulated protein (Pru p 7). We investigated recombinant Pru p 7 (rPru p 7) for its potential use in worldwide standardization for the diagnosis of peach allergy.

    Methods Natural Pru p 7 (nPru p 7) was purified from peach crude extract using a monoclonal antibody affinity column. Complementary DNA for Pru p 7 was cloned and expressed in Escherichia coli and Pichia pastoris. Serum immunoglobulin (Ig) E in peach-allergic patients was examined by enzyme-linked immunosorbent assay (ELISA) using nPru p 7 and rPru p 7 (E. coli product: erPru p 7 and P. pastoris product: prPru p 7).

    Results Peach-allergic patients (n=27) were diagnosed and categorized into oral reaction (n=10) or systemic reaction (n=17). The nPru p 7 positivity based on serum IgE levels was 52% in the systemic-reaction group and 0% in the oral-reaction group (P<0.05). In the systemic-reaction group, there was no significant difference in reactivity between nPru p 7 and prPru p 7, but the reactivity of erPru p 7 was significantly lower than those of nPru p 7 and prPru p 7 (P<0.05).

    Conclusions We found that prPru p 7 exhibited reactivity in ELISA comparable to that of nPru p 7 for the diagnosis of peach allergy with systemic reaction.