An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology.

• Autores: Matthias Georg Haberl, Silvia Viana da Silva, Jason M Guest, Melanie Ginger, Alexander Ghanem, Christophe Mulle, Marcel Oberlaender, Karl Klaus Conzelmann, Andreas Frick
• Localización: Brain Structure and Function, ISSN 1863-2653, ISSN-e 1863-2661, Vol. 220, Nº. 3, 2015, págs. 1369-1379
• Idioma: inglés
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• Resumen

  • Glycoprotein-deleted rabies virus (RABV ∆G) is a powerful tool for the analysis of neural circuits. Here, we demonstrate the utility of an anterograde RABV ∆G variant for novel neuroanatomical approaches involving either bulk or sparse neuronal populations. This technology exploits the unique features of RABV ∆G vectors, namely autonomous, rapid high-level expression of transgenes, and limited cytotoxicity. Our vector permits the unambiguous long-range and fine-scale tracing of the entire axonal arbor of individual neurons throughout the brain. Notably, this level of labeling can be achieved following infection with a single viral particle. The vector is effective over a range of ages (>14 months) aiding the studies of neurodegenerative disorders or aging, and infects numerous cell types in all brain regions tested. Lastly, it can also be readily combined with retrograde RABV ∆G variants. Together with other modern technologies, this tool provides new possibilities for the investigation of the anatomy and physiology of neural circuits.;