Eucommia ulmoidesseed oil is the main byproduct of E. ulmoidescultivation. To better understand its functions, E. ulmoidesseed oil is characterized comprehensively in this work. The composition of E. ulmoidesseed, physicochemical properties, thermal properties, fatty acid composition, triacylglycerol (TAG) composition and Vitamin E composition of E. ulmoidesseed oil were determined. The results show that the E. ulmoidesseed contained about 34.63% oil. The excellent physicochemical properties of E. ulmoidesseed oil ensured it has a potential to be developed as an edible oil. The main fatty acids in E. ulmoidesseed oil were linolenic acid (61.36%), oleic acid (17.02%), and linoleic acid (12.04%). HPLC‐ELSD method determined that LnLnLn (37.99%), LnLnO (22.62%), LnLnL (14.5%), and LnLnP (8.78%) were the oil's major TAG components. The oil exhibited a unique thermal curve which contained 2 melting peaks at –38.45 and –22.22 °C, respectively. The total content of vitamin E in E. ulmoidesseed oil was 190.96 mg/100g, which exist mainly in γ‐tocopherol and δ‐tocopherol isomer. Overall, the results indicated that E. ulmoidesseed oil is a promising oil in food, pharmaceutics, cosmetics and other nonfood industries.
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