A Switchable Linker‐Based Immunoassay for Ultrasensitive Visible Detection of Salmonellain Tomatoes

• Autores: Jungwoo Hahn, Eunghee Kim, Young Sang You, Sundaram Gunasekaran, Seokwon Lim, Young Jin Choi
• Localización: Journal of food science, ISSN 0022-1147, Vol. 82, Nº 10, 2017, págs. 2321-2328
• Idioma: inglés
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• Resumen

  • On‐site detection for sensitive identification of foodborne pathogens on fresh produce with minimal use of specialized instrumentation is crucial to the food industry. A switchable linker (SL)‐based immunoassay was designed for ultrasensitive on‐site detection of Salmonellain tomato samples. The assay is based on large‐scale aggregation of gold nanoparticles (GNPs), induced by a quantitative relationship among the biotinylated Salmonellapolyclonal antibody (b‐Ab) used as the SL, the functionalized GNPs, and Salmonella. Important factors such as the concentration of SLs, time required for large‐scale aggregation, and selectivity of b‐Ab were optimized to minimize the detection time (within 45 min with gentle agitation) and achieve the lowest limit of detection (LOD; 10 CFU/g in tomato samples) possible. This SL‐based immunoassay with its relatively low LOD and short detection time may meet the need for rapid, simple, on‐site analysis of pathogens in fresh produce. The novel switchable linker‐based immunoassay is a rapid, specific, and sensitive method that has potential applications for routine diagnostics of Salmonellain tomato products. These advantages make it a practical approach for general use in the processing industry to detect Salmonella rapidly and to implement appropriate regulatory procedures. Furthermore, it could be applied to other fresh products including cantaloupe, strawberry, and cucumbers.