Exponential growth of extended‐spectrum β‐lactamase (ESBL)‐producing Escherichia coliin Campylobactermedia has become a common problem for the detection of Campylobacterin chicken meats. We investigated the minimum inhibitory concentration of 40 ESBL‐producing E. coliisolates from meats obtained from various countries against antibacterial agents in Bolton broth (cefoperazone, vancomycin, and trimethoprim). All ESBL‐producing E. colistrains were resistant to cefoperazone and vancomycin, whereas 50% of them were resistant to trimethoprim and grew in Bolton broth. We found that 20 μg/mL of rifampicin inhibited the growth of trimethoprim‐resistant E. colistrains. Hence, we added 20 μg/mL of rifampicin to Bolton broth to improve the isolation of Campylobacterfrom chicken carcass rinses. The isolation rate of Campylobacterwas significantly higher in the modified broth (44 out of 58, 75.9%, P< 0.05) than in the normal broth (0 out of 58, 0%). Furthermore, the number of agar plates with non‐Campylobacterspp. was much lower after enrichment in the modified broth (4 out of 58, 6.9%, P< 0.05) than in the normal broth (58 out of 58, 100%).
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