Resumen de Antioxidant Effect of Natural Table Olives Phenolic Extract Against Oxidative Stress and Membrane Damage in Enterocyte‐Like Cells

Gabriele Serreli, Alessandra Incani, Angela Atzeri, Alberto Angioni, Marco Campus, Emanuele Cauli, Roberto Zurru, Monica Deiana

• The phenolic fraction of a naturally fermented cultivar of table olives, “Tonda di Cagliari,” was investigated for the ability to protect Caco‐2 cells against oxidative stress and membrane damage induced by tert‐butyl hydroperoxyde (TBH). TBH exposure resulted in an alteration of cellular redox status, with an increase in reactive oxygen species (ROS) and a decrease in reduced glutathione (GSH) level. A loss of the epithelial integrity, as indicated by the decrease of the transepithelial electrical resistance value, was also observed over time, together with an intense lipid peroxidation process. The olives phenolic extract significantly counteracted ROS generation and subsequent alteration of monolayer integrity and membrane oxidative damage. The protective action of the extract is likely due to the scavenging ability of its main components, as hydroxytyrosol, oleuropein, and verbascoside among the secoiridoids and derivatives. Since olives phenolic compounds concentrate in the intestinal lumen, they may be a useful tool in the prevention of intestinal disorders related to oxidative damage. Naturally fermented table olives of the variety “Tonda di Cagliari” have been found to be rich in hydroxytyrosol, oleuropein and verbascoside. The olive phenolic fraction as a whole protected intestinal cells against oxidative damage, highlighting an added nutraceutical value. Likewise olive oil, they may be considered a good source of active phenolic compounds that may contribute to the maintenance of intestinal mucosal integrity.