High-sensitivity chemiluminescent immunoassay investigation and application for the detection of T-2 toxin and major metabolite HT-2 toxin.

• Autores: Yanshen Li, Jing Zhang, Xin Mao, Yongtao Wu, Gongzhen Liu, Liting Song, Yonggang Li, Jianrong Yang, Yanli You, Xuelin Cao
• Localización: Journal of the science of food and agriculture, ISSN 0022-5142, Vol. 97, Nº 3, 2017, págs. 818-822
• Idioma: inglés
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• Resumen

  • T-2 toxin is a widely distributed mycotoxin in cereals. HT-2 toxin is the major metabolite, which is also a contaminant in cereals. T-2 toxin and HT-2 toxin have been identified as having carcinogenic, hepatotoxic, teratogenic and immunotoxic properties. To reduce the risk of contamination, a rapid, highly sensitive and inexpensive assay for the detection is required.; Results: In this study a high-sensitivity chemiluminescent enzyme-linked immunoassay (CL-ELISA) of T-2 toxin and HT-2 toxin was developed. With the help of the chemiluminescent substrate, this protocol showed a highly sensitive character with an IC50 as low as 33.28 ng mL-1 and 27.27 ng mL-1 for T-2 and HT-2, respectively. In addition, this method had no cross-reaction with other structurally related mycotoxins.; Conclusion: These results indicated that the developed CL-ELISA could be applied for the detection of T-2 toxin and HT-2 toxin in actual samples without complicated steps. © 2016 Society of Chemical Industry.; © 2016 Society of Chemical Industry.