Laminaria digitata phlorotannins decrease protein degradation and methanogenesis during in vitro ruminal fermentation.

• Autores: Anne M Vissers, Wilbert F Pellikaan, Anouk Bouwhuis, Jean-Paul Vincken, Harry Gruppen, Wouter H Hendriks
• Localización: Journal of the science of food and agriculture, ISSN 0022-5142, Vol. 98, Nº 10, 2018, págs. 3644-3650
• Idioma: inglés
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• Resumen

  • Phlorotannins (PhTs) are marine tannins consisting of phloroglucinol subunits connected via carbon-carbon and ether linkages. These have non-covalent protein binding properties and are, therefore, expected to be beneficial in protecting protein from hydrolysis during ruminal fermentation. In this study, the effectiveness of a methanolic PhTs extract from Laminaria digitata (10, 20, 40, 50, 75 and 100 g kg-1 tannin-free grass silage, with or without addition of polyethylene glycol (PEG), was investigated in vitro on protection of dietary protein and reduction of methane (CH4 ) in ruminal fluid.; Results: Addition of PhTs had linear (P < 0.0001) and quadratic (P = 0.0003) effects on gas and CH4 production, respectively. Optimal dosage of PhTs was 40 g kg-1 as at this point CH4 decreased (P < 0.0001) from 24.5 to 15.2 mL g-1 organic matter (OM), without affecting gas production (P = 0.3115) and total volatile fatty acids (P = 1.000). Ammonia trended (P = 0.0903) to decrease from 0.49 to 0.39 mmol g-1 OM, indicating protection of protein. Addition of PEG inhibited the effect of tannins at all dosage levels, and none of the fermentation parameters differed from the control.; Conclusion: PhTs effectively protected protein from fermentation and reduced ruminal methanogenesis. © 2017 Society of Chemical Industry.; © 2017 Society of Chemical Industry.