Resumen de RNA editing of Filamin A pre‐mRNA regulates vascular contraction and diastolic blood pressure
Mamta Jain, Tomer D Mann, Maja Stulić, Shailaja P. Rao, Andrijana Kirsch, Dieter Pullirsch, Xué Strobl, Claus Rath, Lukas Reissig, Kristin Moreth, Tanja Klein‐Rodewald, Raffi Bekeredjian, Valerie Gailus Durner, Helmut Fuchs, Martin Hrabe de Angelis, Eleonore Pablik, Laura Cimatti, David Martín Gálvez, Jelena Zinnanti, Wolfgang F Graier, Maria Sibilia, Saša Frank, Erez Y Levanon, Michael F Jantsch
Epitranscriptomic events such as adenosine‐to‐inosine (A‐to‐I) RNA editing by ADAR can recode mRNAs to translate novel proteins. Editing of the mRNA that encodes actin crosslinking protein Filamin A (FLNA) mediates a Q‐to‐R transition in the interactive C‐terminal region. While FLNA editing is conserved among vertebrates, its physiological function remains unclear. Here, we show that cardiovascular tissues in humans and mice show massive editing and that FLNA RNA is the most prominent substrate. Patient‐derived RNA‐Seq data demonstrate a significant drop in FLNA editing associated with cardiovascular diseases. Using mice with only impaired FLNA editing, we observed increased vascular contraction and diastolic hypertension accompanied by increased myosin light chain phosphorylation, arterial remodeling, and left ventricular wall thickening, which eventually causes cardiac remodeling and reduced systolic output. These results demonstrate a causal relationship between RNA editing and the development of cardiovascular disease indicating that a single epitranscriptomic RNA modification can maintain cardiovascular health.
