Resumen de Effect of phytase dose on productive performance and bone status of layers fed with graded levels of digestible lysine
Ingrid Y. Martinez Rojas, Carlos López Coello, E. Ávila González, José Arce Menocal, G.A. Gomes
Exogenous phytase could influence dietary protein availability by variation in using dose. The objective of the present study was to determine if incremental dosing of an evolved E.coli 6-phytase would lead to improvements in lysine availability through evaluating performance and bone status in Bovans White layers. A total of 182 layers were placed in individual cages and distributed to 13 treatments: a 3 × 4 factorial arrangement with three levels of digestible lysine (dLys - 0.67, 0.77, and 0.87 %) and four doses of phytase (0, 300, 1 200, and 4 800 FTU/kg) in 0.12 % available P (avP) diets. Additionally, one phytase-free control treatment was included with 0.25 % avP and 0.87 % dLys. Productive parameters were recorded for 25 weeks, from 39 week-old. At the end, abdominal fat deposition and the tibia were sampled; in bone was determined breaking strength and bone ash. Layers fed 1 200 FTU/kg phytase increased egg production percentage (F3,169 = 2.01, p = 0.111), abdominal fat deposition (F3,169 = 2.52, p = 0.059), bone breaking strength (F3,169 = 4.29, p = 0.006) and bone ash weight (F3,169 = 3.62, p = 0.014) compared with non-phytase inclusion.
Furthermore, 1 200 FTU/kg phytase decreased incidence of broken eggs and soft-shell eggs (F3,169 = 2.9, p = 0.036). Phytase and dLys levels influenced egg mass and bone ash concentration (F12,169 = 1.86, p = 0.043).
FCR and body weight loss was reduced with phytase inclusion (respectively:
F12,169 = 2.43, p = 0.006, and F12,169 = 2.24, p = 0.012). Phytase-free control diet increased egg weight (F12,169 = 3.70, p < 0.001), but gave greater BW loss (F12,169 = 17.79, p < 0.001), less abdominal fat content (F12,169 = 5.85, p < 0.001), and no effect on other variables (p > 0.07).
In conclusion, 1 200 FTU/kg of phytase improved productive performance and preserved body weight and bone status, without equivalence of phytase inclusion for dLys level, even with higher doses.
