Effect of resveratrol on the in vitromaturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos

José Luis Martínez Ibarra; Eugenia Adriana Espinoza Mendoza; Raymundo Rangel Santos; Demetrio Alonso Ambriz Garcia; María del Carmen Navarro Maldonado

Ayuda

Effect of resveratrol on the in vitromaturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos

José Luis Martínez-Ibarra ^[1] ; Eugenia Adriana Espinoza-Mendoza ^[1] ; Raymundo Rangel-Santos ^[2] ; Demetrio Alonso Ambriz-Garcia ^[1] ; María del Carmen Navarro-Maldonado ^[1]
1. [1] Universidad Autónoma Metropolitana
  
  Universidad Autónoma Metropolitana
  
  México
2. [2] Universidad Autónoma Chapingo
  
  Universidad Autónoma Chapingo
  
  México
Localización: Veterinaria México, ISSN 0301-5092, Vol. 5, Nº. 4, 2018, págs. 12-26
Idioma: varios idiomas
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Resumen
- The effect of resveratrol on the in vitro maturation (IVM) of ovine (Ovis aries) oocytes and the development of handmade cloned embryos was eval-uated. The nuclear maturation and reactive oxygen species (ROS) levels in the oocytes, as well as the early development and morphological cloned embryo quality, were evaluated under different resveratrol concentrations (0, 0.5, 2 and 5μM). After IVM, no significant difference was observed in the maturation rate of oocytes treated with 0.5μM (81.3%) and 2μM (72%) resveratrol compared to that of the control group (0μM) (74.2%), but the rate significantly decreased at 5μM (56%) (p<0.05). When the oocyte ROS levels were determined, no significant differences among the groups were observed (p>0.05). For cloned embryo development, the embryos obtained from the oocytes treated with 0.5μM resveratrol showed higher (p<0.05) compacted morula rates (10.7%) compared to the embryos obtained from the oocytes treated with 0, 2 and 5μM (6.2, 0 and 0%, re-spectively). Regarding embryo morphological quality, the embryos from the oocytes treated with 0.5μM resveratrol showed a lower rate of poor quality morulae (4.7%) in comparison to those treated with 0, 2 and 5μM (23.8, 23.3 and 33.3%, respectively) (p<0.05). In conclusion, resveratrol showed no significant improvement on the IVM or ROS levels in domestic ovine oocytes. However, treatment with 0.5μM resveratrol during IVM improved embryo quality and promoted morulae compaction of Ovis aries handmade cloned embryos.