In vitro evaluation of probiotic strains for lactic acid production

• Ahmed Faraz ^[1] ; Rao Arathi ^[2] ; Saralaya Vishwas ^[3] ; Natarajan Srikanth ^[4] ; Yarmunja-Mahabala Karuna ^[5] ; Baranya-Srikrishna Suprabha ^[6]
  1. [1] Ex Post Graduate Student. Department of Paedodontics and Preventive Dentistry Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal
  2. [2] Professor. Department of Paedodontics and Preventive Dentistry Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal
  3. [3] Associate Professor. Department of Microbiology Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal
  4. [4] Professor. Department of Oral Pathology Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal
  5. [5] Assistant Professor. Department of Paedodontics and Preventive Dentistry Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal
  6. [6] Professor & Head. Department of Paedodontics and Preventive Dentistry Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal

  Mostrar afiliaciones +
• Localización: Journal of Clinical and Experimental Dentistry, ISSN-e 1989-5488, Vol. 11, Nº. 4 (April ), 2019, págs. 340-345
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • The growing interest on usage of probiotic lactobacilli in maintaining oral health has posed number of questions on its probable side effects. One such consideration could be an increased acid production in dental plaque, in turn leading to dental caries. Thus, the aim of this study was to comparatively evaluate the lactic acid producing ability of L. acidophilus and L. plantarum with and without dental plaque.

    The study consisted of five groups: 3 control groups (Supragingival plaque, L. acidophilus and L. plantarum) and 2 test groups (Supragingival plaque with L. acidophilus and Supragingival plaque with L. plantarum). 26 samples for each group were collected and their baseline spectrophotometric values were recorded. The acid production was initiated by adding 25?l fructose (10%) and stopped by centrifugation for 2 min. The concentration of the lactic acid produced was determined with the aid of COBAS INTEGRA 400 plus.

    On comparison of Lactic Acid estimation in mg/dl, the mean values of Plaque group was the highest followed by Plaque +L acidophilus, Plaque +L plantarum, L acidophilus and least in L plantarum. The posthoc analysis shows that the comparison of Group 1 (Plaque) and Group 2 (Plaque +L acidophilus) is statistically Significant results between all the groups except between the Plaque +L acidophilus and Plaque +L plantarum group.

    The lactic acid producing ability of pure suspensions of L.acidophilus and L.plantarum and the lactic acid producing efficiency becomes more when they are added to the supragingival plaque.