Colonization of "Rubus glaucus Benth" by azorhizobium caulinodans ors571 usig a flavonoide naringenina

• Cancino Escalante, Giovani Orlando ^[1] ; Cancino, S E ^[1] ; Quevedo Garcia, Enrique
  1. [1] Universidad de Pamplona

     Universidad de Pamplona

     Colombia

     
• Localización: Bistua: Revista de la Facultad de Ciencias Básicas, ISSN 0120-4211, Vol. 14, Nº. 2, 2016, págs. 15-26
• Idioma: inglés
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• Resumen

  • Root systems of two Andean blackberry materials (thorn and thornless) of Rubus glaucus Benth cultured in vitro in the presence of five treatments (four flavonoids and one control) were inoculated with Azorhizobium caulinodans ORS571 (pXLGD4)  (a strain carrying the lacZ reporter gene which facilitated the detection of bacterial colonization). Evaluation of colonization effectiveness for each treatment was done by means of application of experimental design measuring frequency and intensity parameters. Statistical analysis showed differences at comparing flavonoids vs. control and the overall higher effectiveness of the flavonoid naringenin. Observation of colonization was made by light and electron microscope confirming internal colonization of Andean blackberry roots by A. caulinodans. This is the first work demonstrating root colonization of R.glaucus by azorhizobia and therefore settling the basis for future investigations and scientific applications related to interaction with plant growth-promoting bacteria under the effect of flavonoids, along with possible implications of common benefit for non-legume crops in the northwest region of Colombia.  Key Words: Azorhizobium caulinodans ORS571, Andean blackberry, flavonoids, LacZ, lateral roots, naringenin.