Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing

• Timothy Tapscott ^[1] ; Michael T. Guarnieri ^[1] ; Calvin A. Henard ^[1]
  1. [1] National Renewable Energy Laboratory

     National Renewable Energy Laboratory

     Estados Unidos

     
• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 11, 2019
• Idioma: inglés
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• Resumen

  • In this study, we targeted the development and evaluation of broad-host-range CRISPR/Cas9 gene-editing tools in order to enhance the genetic-engineering capabilities of an industrially relevant methanotrophic biocatalyst. The CRISPR/Cas9 system developed in this study expands the genetic tools available to define molecular mechanisms in methanotrophic bacteria and has the potential to foster advances in the generation of novel biocatalysts to produce biofuels, platform chemicals, and high-value products from natural gas- and biogas-derived methane. Further, due to the broad-host-range applicability, these genetic tools may also enable innovative approaches to overcome the barriers associated with genetically engineering diverse, industrially promising nonmodel microorganisms.