Pleiotropic Effects of c-di-GMP Content in Pseudomonas syringae

• Tingting Wang ^[1] ; Zhao Cai ^[2] ; Xiaolong Shao ^[3] ; Weitong Zhang ^[1] ; Yingpeng Xie ^[1] ; Yingchao Zhang ^[3] ; Canfeng Hua ^[1] ; Stephan C. Schuster ^[4] ; Liang Yang ^[5] ; Xin Deng ^[1]
  1. [1] City University of Hong Kong

     City University of Hong Kong

     RAE de Hong Kong (China)

     
  2. [2] Nanyang Technological University

     Nanyang Technological University

     Singapur

     
  3. [3] Nankai University

     Nankai University

     China

     
  4. [4] Singapore Centre for Environmental Life Sciences Engineering

     Singapore Centre for Environmental Life Sciences Engineering

     Singapur

     
  5. [5] b Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore; d School of Medicine, Southern University of Science and Technology (SUSTec), Shenzhen, Guangdong, China

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• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 10, 2019
• Idioma: inglés
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• Resumen

  • The present work comprehensively analyzed the transcriptome and phenotypes that were regulated by c-di-GMP in P. syringae. Given that the majority of diguanylate cyclases and phosphodiesterases have not been characterized in P. syringae, this work provided a very useful database for the future study on regulatory mechanism (especially its relationship with T3SS) of c-di-GMP in P. syringae. In particular, we identified three promoters that were sensitive to elevated c-di-GMP levels and inserted them into luciferase-based reporters that effectively respond to intracellular levels of c-di-GMP in P. syringae, which could be used as an economic and efficient way to measure relative c-di-GMP levels in vivo in the future.