Development of Aspirin-Inducible Biosensors in Escherichia coli and SimCells

• Jack Xiaoyu Chen ^[1] ; Harrison Steel ^[1] ; Yin-Hu Wu ^[1] ; Yun Wang ^[1] ; Jiabao Xu ^[1] ; Cordelia P. N. Rampley ^[1] ; Ian P. Thompson ^[1] ; Antonis Papachristodoulou ^[1] ; Wei E. Huang ^[1]
  1. [1] University of Oxford

     University of Oxford

     Oxford District, Reino Unido

     
• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 6, 2019
• Idioma: inglés
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• Resumen

  • An aspirin-inducible SalR/Psal regulation system, originally from Acinetobacter baylyi ADP1, has been designed for E. coli strains. SalR is a typical LysR-type transcriptional regulator (LTTR) family protein and activates the Psal promoter in the presence of aspirin or salicylate in the range of 0.05 to 10 µM. The experimental results and mathematical simulations support the competitive binding model of the SalR/Psal regulation system in which SalRr competes with SalRa to bind the Psal promoter and affect gene transcription. The competitive binding model successfully predicted that weak SalR expression would significantly improve the inducible strength of the SalR/Psal regulation system, which is confirmed by the experimental results. This provides an important mechanism model to fine-tune transcriptional regulation of the LTTR family, which is the largest family of transcriptional regulators in the prokaryotic kingdom. In addition, the SalR/Psal regulation system was also functional in probiotic strain E. coli Nissle 1917 and minicell-derived SimCells, which would be a useful biobrick for environmental and medical applications.