Posttranslational Targeting of a Recombinant Protein Promotes Its Efficient Secretion into the Escherichia coli Periplasm

• A. Jimmy Ytterberg ^[3] ; Roman A. Zubarev ^[1] ; Thomas Baumgarten ^[2]
  1. [1] Karolinska Institute

     Karolinska Institute

     Suecia

     
  2. [2] Royal Institute of Technology

     Royal Institute of Technology

     Suecia

     
  3. [3] a Chemistry I Division, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden; b Rheumatology Unit, Department of Medicine, Karolinska Institute, Stockholm, Sweden

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• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 13, 2019
• Idioma: inglés
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  • The bacterium Escherichia coli is widely used to produce recombinant proteins. To fold properly, many recombinant proteins have to be targeted to the E. coli periplasm, but so far the impact of the targeting pathway of a recombinant protein to the periplasm has not been extensively investigated. Here, we show that the targeting pathway of a recombinant antibody fragment has a tremendous impact on cell physiology, ultimately affecting protein production yields in the periplasm and biomass formation. This indicates that studying the targeting and secretion of proteins into the periplasm could be used to design strategies to improve recombinant protein production yields.