Background: This study aimed to investigate the accuracy and reliability of a free mobile heart rate variability (HRV) application in measuring HRV. Methods: Twelve females and 12 males underwent five-minute simultaneous HRV recording from electrocardiogram (ECG) and chest strap connected to a free mobile HRV application (HRVapp) in a supine position. HRV data from ECG and HRVapp were used to examine accuracy and reliability via relative error and intraclass correlation coefficient respectively. Results: The natural log of the square root of the mean of the sum of the squares of differences between adjacent normal to normal intervals (lnRMSSD) exhibited accuracy and high reliability in HRVapp. Conclusion: lnRMSSD in HRVapp can serve as an alternative, low-cost technology for measurement of autonomic activity.
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