Resumen de Development and Validation of a Method Using Dried Oral Fluid Spot to Determine Drugs of Abuse
Ana Laura Bemvenuti Jacques, Maíra Kerpel dos Santos, Renata Pereira Limberger
A liquid chromatography–mass spectrometry method using dried oral fluid spots was developed and validated for the simulta- neous quantification of cocaine, benzoylecgonine, cocaethylene, amphetamine, and 3,4-methylenedioxymethamphetamine. The oral fluid was applied to a Whatman 903 grade paper and submitted to a drying time of 2.5 h. The extraction procedure was optimized by chemometric approach using simplex centroid design. Spots were extracted with a mixture of acetonitrile, buffer, and methanol. Calibration curves covered a linear concentration range of 40–500 ng/mL. Validation parameters of linearity, precision, accuracy, selectivity, carryover, matrix effects, and stability were evaluated and showed satisfactory results. Spot homogeneity was also satisfactory, with less than 15% of deviation from nominal concentration. Spot volume did not influence accuracy when less than 100 lL of the sample was applied to the spot. The validation of the proposed method suggests a potential application in different scenarios in toxicology.
