Effectiveness of different disinfection techniques of the root canal in the elimination of a multi-species biofilm

• Abel Teves ^[1] ; Daniel Blanco ^[2] ; Mario Casaretto ^[3] ; John Torres ^[5] ; Debora Alvarado ^[3] ; David E. Jaramillo ^[4]
  1. [1] Universidad Inca Garcilaso de la Vega

     Universidad Inca Garcilaso de la Vega

     Perú

     
  2. [2] Universidad Peruana Cayetano Heredia

     Universidad Peruana Cayetano Heredia

     Perú

     
  3. [3] Universidad Nacional Mayor de San Marcos

     Universidad Nacional Mayor de San Marcos

     Perú

     
  4. [4] University of Texas Health Science Center at Houston

     University of Texas Health Science Center at Houston

     Estados Unidos

     
  5. [5] Universidad Catolica Santo Toribio de Mogroviejo

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• Localización: Journal of Clinical and Experimental Dentistry, ISSN-e 1989-5488, Vol. 11, Nº. 11 (November), 2019, págs. 978-983
• Idioma: inglés
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• Resumen

  • The purpose of the study was to evaluate the effectiveness of different root canal disinfection techniques in the elimination of a multi-species biofilm from inside the root canal.

    Fifty mandibular first premolars were used in the present study, standardized to 11mm of root length, and instrumented with a reciprocation system Reciproc, (VDW GmbH, Munich, Germany) to a #50. Longitudinally sectioned halves of the roots were obtained and washed with NaOCl 4%, EDTA 17% and 5% sodium thiosulfate, and sterilized by autoclaving for 15 minutes at 121°C. A multi-species biofilm broth was developed with three strains of bacteria under laboratory conditions: Enterococcus faecalis ATTC 29212, Eikenella corrodens ATTC 23834, Streptococcus anginosus ATTC 33397. Roots were autoclaved and transferred to the broth for 4 days and then were subjected to either disinfection with sodium hypochlorite 4% and XP-endo Finisher (FKG Dentaire, La Chaux-de-Fonds, Switzerland) or chlorhexidine 2% with and without activation with XP-endo Finisher (FKG Dentaire, La Chaux-de-Fonds, Switzerland).

    The evaluations of the biofilm elimination showed results that indicate that the 4% sodium hypochlorite group with positive pressure irrigation presented significant differences with the group that had irrigation with sodium hypochlorite activated with XP-endo Finisher and the chlorhexidine groups to 2% (P<0.05).

    Chlorhexidine 2% activated with the XP-endo Finisher does not exert elimination or improved cleaning effect on the multi-species biofilm. Activation of sodium hypochlorite 4% improved the elimination of the multi-species biofilm.