Resumen de Comparative analysis of the remineralization potential of CPP–ACP with Fluoride, Tri-Calcium Phosphate and Nano Hydroxyapatite using SEM/EDX – An in vitro study
Charisma Thimmaiah, Priya Shetty, Sowmya B. Shetty, Srikant Natarajan, Nithya-Annie Thomas
In recent years, the non-invasive management of non cavitated caries lesions using remineralization systems to repair the enamel have received more attention from the scientific community. Aim: To quantitatively evaluate the remineralization potential of Casein phosphopeptide-amorphous calcium phosphate-fluoride(CPP-ACPF), Tri-calcium phosphate(TCP) & Nano-hydroxyapatite(nHAP) using Scanning Electron Microscopy(SEM) and Energy dispersive X-ray Analysis(EDX).
40 enamel specimens were prepared, and immersed in demineralising solution at a pH of 4.4 for 96 hours at 37°C, to induce artificial carious lesions. Remineralization was carried out for a period of 30 days using CPP-ACPF, TCP, nHAP. The specimens were evaluated for calcium and phosphorus content using SEM-EDX.
The Ca/P mass % after remineralization was significantly higher with CPP-ACP-F and TCP-F followed by nHAP.
CPP-ACP-F and TCP can promote significant remineralization of incipient carious lesions. These are excellent delivery vehicles available in a slow release amorphous form to localize calcium, phosphate and fluoride at the tooth surface.
