2P1, a novel male mouse cDNA specifically expressed during meiosis

• Autores: Mireille Doussau, Jean-Marie Gasc, Sonia Hammami-Hamza, Isabelle Allemand, Dominique Segretain, Catherine Finaz
• Localización: International journal of developmental biology, ISSN 0214-6282, Vol. 47, Nº. 1, 2003, págs. 71-76
• Idioma: inglés
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• Resumen

  • We screened a mouse germinal cell expression library with a probe derived from Sob1, a human testis-specific cDNA, and identified 2P1, a new mouse cDNA. A database search revealed that 2P1 was 91% identical to ORF1 of E3-3, a rat gene probably involved in the regulation of alternative splicing. Sequencing showed that 2P1 has a destabilization motif in its 3'-untranslated region. Northern blotting showed strong gene expression in the testis and weak expression in the epididymis, with no signal detected in other tissues. RT-PCR analysis confirmed testis and epididymis expression. In situ hybridization revealed that 2P1 mRNA was absent in spermatogonia but expressed in spermatocytes. This last result was confirmed by RT-PCR of FACS isolated primary spermatocytes (pachytene stage). Using RT-PCR, purified spermatids were also shown to express 2P1.