In Situ Activation and Heterologous Production of a Cryptic Lantibiotic from an African Plant Ant-Derived Saccharopolyspora Species

• Eleni Vikeli ^[4] ; David A. Widdick ^[1] ; Sibyl F. D. Batey ^[1] ; Daniel Heine ^[1] ; Neil A. Holmes ^[2] ; Mervyn J. Bibb ^[1] ; Dino J. Martins ^[3] ; Naomi E. Pierce ^[3] ; Matthew I. Hutchings ^[2] ; Barrie Wilkinson ^[1]
  1. [1] John Innes Centre

     John Innes Centre

     Norwich District, Reino Unido

     
  2. [2] University of East Anglia

     University of East Anglia

     Norwich District, Reino Unido

     
  3. [3] Harvard University

     Harvard University

     City of Cambridge, Estados Unidos

     
  4. [4] a Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom; b School of Biological Sciences, University of East Anglia, Norwich, United Kingdom

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• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 86, Nº 3, 2020
• Idioma: inglés
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• Resumen

  • The discovery of novel antibiotics to tackle the growing threat of antimicrobial resistance is impeded by difficulties in accessing the full biosynthetic potential of microorganisms. The development of new tools to unlock the biosynthesis of cryptic bacterial natural products will greatly increase the repertoire of natural product scaffolds. Here, we report a strategy for the ectopic expression of pathway-specific positive regulators that can be rapidly applied to activate the biosynthesis of cryptic lanthipeptide biosynthetic gene clusters. This allowed the discovery of a new lanthipeptide antibiotic directly from the native host and via heterologous expression.