Development of 21 Microsatellite Loci and Diversity Analysis of Amur Grayling in Amur River
-
Haicheng Sun [1] ; Lina Zhang [1] ; Shuqun Xue [1] ; Ying Han [1] ; Xiaonan Lv [2] ; Youyi Kuang [3] ; Guangxiang Tong [3] ; Jiasheng Yin [3]
-
[1] Northeast Agricultural University
Northeast Agricultural University
China
-
[2] Beijing Fisheries Technical Extension Station, China
-
[3] Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, China
-
- Localización: Thalassas: An international journal of marine sciences, ISSN 0212-5919, Vol. 36, Nº. 1, 2020, págs. 165-170
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
-
Resumen
Amur grayling (Thymallus arcticus grubei Dybowski), a cold freshwater fish, is an economically valuable species that is endemic to Heilongjiang Province, China. In this study, we developed 21 polymorphic microsatellite loci obtained from transcriptome data, and genetically analyzed 66 individuals from three natural riverine populations. The genetic diversity analysis showed that the number of alleles per locus ranged from 2 to 12 with mean value of 4.7. The expected heterozygosity ranged from 0.0800 to 0.9400 with mean value of 0.4339, while the observed heterozygosity ranged from 0.0960 to 0.8832 with mean value of 0.5243. The polymorphism information content (PIC) varied from 0.8509 to 0.0380, with an average value of 0.52. The Hardy-Weinberg equilibrium (HWE) test showed that most of loci were complied with HWE. The population genetic structure inferred with Bayesian method showed that the three Amur populations was divided into two main groups, and the genetic intervals are mainly positively correlated with geographical intervals. These transcript-derived microsatellite markers and the genetic diversity analysis could be promising for conservation of Amur grayling.
