Enhancement of Sphingolipid Synthesis Improves Osmotic Tolerance of Saccharomyces cerevisiae

Guoxing Zhu ^[1] ; Nannan Yin ^[1] ; Qiuling Luo ^[1] ; Jia Liu ^[1] ; Xiulai Chen ^[1] ; Liming Liu ^[1] ; Jianrong Wu ^[2]
1. [1] Jiangnan University
  
  Jiangnan University
  
  China
2. [2] b Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China; c Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 86, Nº 8, 2020
Idioma: inglés
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Resumen
- This study demonstrated a novel strategy for the manipulation of membrane complex sphingolipids to enhance S. cerevisiae tolerance to osmotic stress. Elo2, a sphingolipid acyl chain elongase, was related to osmotic tolerance through transcriptome analysis of the wild-type strain and an osmosis-tolerant strain generated from ALE. Overexpression of ELO2 increased the content of complex sphingolipid with longer acyl chain; thus, membrane integrity and osmotic tolerance improved.