Micro-morphological and molecular study of four species of Lonicera (Caprifoliaceae) in Iran

• Autores: Elham Amini, Fatemeh Nasrollahi, Ali Sattarian, Abdolsaber Khormali, Meisam Habibi
• Localización: Phytologia Balcanica: International Journal of Balkan Flora and Vegetation, ISSN 1310-7771, Vol. 25, Nº. 2, 2019, págs. 181-190
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Lonicera is one of the genera of Caprifoliaceae presented with nine species in Iran. In this study, the micromorphological and molecular characters of 12 populations from four species of Lonicera (L. bracteolaris, L.

    hypoleuca, L. iberica and L. korolkowii) have been analyzed so as to evaluate their diagnostic value. Seven quantitative and qualitative characters of pollen were selected and measured. The most important characters include: shape, ornamentation of tectum, exine thickness, and P/E ratio of the pollen. On the basis of this study, the seed shape and surface contribute at least to differentiation of these species. Using nuclear (nrDNA ITS) markers, phylogenetic relationships within the four species of Lonicera have been reconstructed. Then the data set was analyzed by phylogenetic methods including Bayesian, Maximum Likelihood, and Maximum Parsimony methods. In phylogenetic analyses, all members of the four species formed a well-supported clade (PP=1, ML/BS=100/100) and divided into three major clades (I, II and III). The Neighbor Net Diagram supported the phylogenetic results. The results showed that micro-morphological and molecular data provide reliable evidence for differentiation of some populations from others.